EFFICIENT PLANT REGENERATION OF MALAYSIAN AROMATIC RICE (ORYZA SATIVA L.) VIA IMPROVED SOMATIC EMBRYOGENESIS PATHWAY

Zuraida Abdul Rahman; Asfaliza Ramli; Rahiniza Kamaruzaman; Zulkifli Ahmad Seman; Ayu Nazreena Othman; Zamri Zainal; Jasim Uddain; Sreeramanan Subramaniam

doi:10.9755/ejfa.2015-07-535

Authors

Zuraida Abdul Rahman Biotechnology Research Centre, MARDI Headquarters, Persiaran MARDI-UPM Serdang, Selangor Malaysia;
Asfaliza Ramli Rice Industry Crop Centre, MARDI Headquarters, Persiaran MARDI-UPM Serdang, Selangor Malaysia
Rahiniza Kamaruzaman Rice Industry Crop Centre, MARDI Headquarters, Persiaran MARDI-UPM Serdang, Selangor Malaysia
Zulkifli Ahmad Seman Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor;
Ayu Nazreena Othman Biotechnology Research Centre, MARDI Headquarters, Persiaran MARDI-UPM Serdang, Selangor Malaysia;
Zamri Zainal Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor
Jasim Uddain School of Biological Sciences, Universiti Sains Malaysia, 11800 Gelugor, Penang, Malaysia
Sreeramanan Subramaniam School of Biological Sciences, Universiti Sains Malaysia, 11800 Gelugor, Penang, Malaysia

DOI:

https://doi.org/10.9755/ejfa.2015-07-535

Keywords:

Aromatic rice, Somatic embryogenesis, Plant growth regulator, Carbon source

Abstract

Aromatic rice MRQ 74, MRQ 80 and MRQ 50 are the most popular rice in Malaysia. Establishing an efficient regeneration protocols via somatic embryogenesis is required for varietal improvements. Somatic embryogenesis system was established in three aromatic rice varieties by using mature seeds. Optimised MS-B5 medium containing 1 mg/L 2, 4-D (2, 4-dichlorophenoxyacetic acid) and 10 mg/L NAA (α-naphthaleneacetic acid) produced highest production of embryogenic callus (89%) without any browning effect. The highest whitish somatic embryos frequency (75 %) was initiated by incubating embryogenic calli on same media containing 10 mg/L ABA and 9 g/L Gelrite agar for 8 weeks. However, the numbers of regenerated plantlets on medium containing NAA and 2,4-D which was previously pre-treated with 10 mg/L ABA, 9 g/L Gelrite agar and incubation at 8 weeks was the best method for shoots induction (14 plantlets per 3 g of somatic embryos) of three rice varieties.