Optimizing the making of modified plantain flour by the mixed-culture of lactic acid bacteria and yeast

Authors

  • Fitri Setiyoningrum Research Center for Applied Microbiology, National Research and Innovation Agency, Indonesia. Jl
  • Gunawan Priadi Research Center for Applied Microbiology, National Research and Innovation Agency, Indonesia. Jl
  • Fifi Afiati Research Center for Applied Microbiology, National Research and Innovation Agency, Indonesia. Jl
  • Dewi Desnilasari Research Center for Applied Microbiology, National Research and Innovation Agency, Indonesia. Jl
  • Rima Kumalasari Research Center for Appropriate Technology, National Research and Innovation Agency, Indonesia. Jl. K.S. Tubun No. 5 Subang, 41213 West Java, Indonesia
  • Riyanti Ekafitri Research Center for Appropriate Technology, National Research and Innovation Agency, Indonesia. Jl. K.S. Tubun No. 5 Subang, 41213 West Java, Indonesia

DOI:

https://doi.org/10.9755/ejfa.2023.3181

Abstract

Fermentation factors in the making of modified plantain flour must be optimized to obtain optimum conditions that produce the desired characteristics of flour/starch. In this research, we used mix-cultured lactic acid bacteria (LAB) and yeast for plantain fermentation. Response Surface Methodology (RSM) was used to explain the quantitative relationship between input and response variables. The design used is Combine D-optimal. The quantitative independent variables (X) selected were A = culture concentration (lower limit 2%; upper limit 6%) and B = fermentation time (lower limit 8 hours; upper limit 24 hours). Descriptive variables were C = type of plantain (C1 = “tanduk” plantain; C2 = “nangka” plantain) and D = type of culture (D1 = SC; D2 = LAB1 + LAB2; D3 = LAB1 + LAB3; D4 = LAB2 + LAB3; D5 = LAB1 + LAB1 + SC; D6 = LAB1 + LAB3 + SC; D7 = LAB2 + LAB3 + SC). Parameters of flour characteristic analyzed or response (Y) were carboxyl number (Y1), total acid (Y2), swelling number (Y3), pH (Y4), paste clarity (Y5), gelatinization properties: peak (Y6); trough 1 (Y7); breakdown (Y8); final viscosity (Y9); setback(Y10); peak time (Y11); pasting temperature (Y12), water content (Y13), color : *L (Y14); *a(Y15); *b (Y16) and c (Y17). The result showed that a combination of 6% culture concentration and 12.62 hours of fermentation time with a desirability value of 0.66 was the optimum condition with carboxyl value 0.15, total acid 0.47, swelling rate 13.49, peak time 5397.02, breakdown 2259.22, final viscosity 4125.22, setback 98.99, and dL 65.59.

Keywords: modification flour, optimum condition, fermentation

 

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Published

2023-12-12

How to Cite

Setiyoningrum, F., G. Priadi, F. Afiati, D. Desnilasari, R. Kumalasari, and R. Ekafitri. “Optimizing the Making of Modified Plantain Flour by the Mixed-Culture of Lactic Acid Bacteria and Yeast”. Emirates Journal of Food and Agriculture, vol. 35, no. 11, Dec. 2023, doi:10.9755/ejfa.2023.3181.

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Section

Research Article