OPTIMATION OF ENRICHMENT AND PURIFICATION OF POLYPHENOLS FROM BLUEBERRIES (VACCINIUM SPP.) BY MACROPOROUS RESINS XAD-7HP

Xinyao Jiao; Bin Li; Xiuyan Zhang; Qi Zhang; Ningxuan Gao; Xianjun Meng

doi:10.9755/ejfa.2017-02-365

Authors

Xinyao Jiao College of Food Science, Shenyang Agricultural University, Dongling Road 120, Shenhe District, Shenyang, China
Bin Li College of Food Science, Shenyang Agricultural University, Dongling Road 120, Shenhe District, Shenyang, China
Xiuyan Zhang College of Food Science, Shenyang Agricultural University, Dongling Road 120, Shenhe District, Shenyang, China
Qi Zhang College of Food Science, Shenyang Agricultural University, Dongling Road 120, Shenhe District, Shenyang, China
Ningxuan Gao College of Food Science, Shenyang Agricultural University, Dongling Road 120, Shenhe District, Shenyang, China
Xianjun Meng College of Food Science, Shenyang Agricultural University, Dongling Road 120, Shenhe District, Shenyang, China

DOI:

https://doi.org/10.9755/ejfa.2017-02-365

Keywords:

Blueberry (Vaccinium spp.), polyphenol, macroporous resin XAD-7HP, purification, cellular antioxidant activity (CAA)

Abstract

Introduction: Blueberries are rich sources of phenolic compounds and exhibit strong antioxidant activities and a wide variety of biological activities.
Methods: Polyphenols were extracted from blueberry by solvent extraction method using ethanol solution. The purification of polyphenols from blueberries (Vaccinium spp.) by macroporous resin XAD-7HP was optimized by adsorption and desorption tests. The cellular antioxidant activities of the purified polyphenols were investigated.
Results: The adsorption data indicated that the process was best explained by the Langmuir isotherm model. The best conditions were determined as: blueberry polyphenols concentration, 2.00 mg/g; pH 2.0; ethanol desorption solution concentration, 70 % (v/v); flow rates for feeding and elution, 1 and 2 bed volume (BV)/h, respectively; and a water wash volume of 3 BV. Under these conditions, the purity of the blueberry polyphenols was improved from 46.3 to 86.48 %. The EC50 (concentration for 50 % of maximal effect) and CAA (cellular antioxidant activity) values of the blueberry polyphenols were 151.58 mg/mL and 6.39 µmol QE /100 g sample, respectively.
Conclusion: The results demonstrate that purification of polyphenols by macroporous resins XAD-7HP will provide foundation for the development of large-scale separation of blueberry polyphenols in industry.