PHYTASE PRODUCTION BY CANDIDA MELIBIOSICA 2491 ALKALOPHYLIC STRAIN

Danail Georgiev; Velichka Gotcheva; Angel Angelov; Aleksandar Slavchev; Stoyanka Gargova

doi:10.9755/ejfa.v25i5.13633

Authors

Danail Georgiev Department of Biochemistry and Microbiology, University of Plovdiv, 21 Kostaki Peev Str., 4000 Plovdiv, Bulgaria
Velichka Gotcheva Department of Biotechnology, University of Food Technology, 26 Maritza blvd., 4002 Plovdiv, Bulgaria
Angel Angelov 2 Department of Biotechnology, University of Food Technology, 26 Maritza blvd., 4002 Plovdiv, Bulgaria 3
Aleksandar Slavchev 3 Department of Microbiology, University of Food Technology, 26 Maritza blvd., 4002 Plovdiv, Bulgaria
Stoyanka Gargova 2 Department of Biotechnology, University of Food Technology, 26 Maritza blvd., 4002 Plovdiv, Bulgaria

DOI:

https://doi.org/10.9755/ejfa.v25i5.13633

Keywords:

Alkalophylic strain, Candida melibiosica, Phytase activity

Abstract

It has been proved that Candida melibiosica 2491 yeast strain, an intracellular phytase producer, can be well adapted to a nutritious medium with a pH range from 3.0 to 9.0. The best development of the strain was achieved at pH 8.0, which refers to the rarely encountered alkalophylic yeast strains. The maximum biosynthesis was obtained in a medium with pH of 8.5, 24th?hour aged inoculum added at concentration of 6.6% (v/v) with optical density OD600 of 0.68, aeration degree 500 dm3 /dm3 .h, stirring speed 300 rpm, and 24h duration of the cultivation in bioreactor. The optimal phytase activity was reached at 60°? and ?? 4.5. However, over 80% of the maximum enzymatic activity was observed at the pH interval ranging from 4.0 to 8.5, which provides great potential for practical applications.