ESTIMATION OF ANTIOXIDANT ACTIVITY OF DIFFERENT MIXED HERBAL INFUSIONS USING ATTENUATED TOTAL REFLECTANCE FOURIER TRANSFORM INFRARED SPECTROSCOPY AND CHEMOMETRICS

Aikaterini Venetsanou; Eirini Anastasaki; Chrysavgi Gardeli; Petros A. Tarantilis; Christos S Pappas

doi:10.9755/ejfa.2016-11-1670

Authors

Aikaterini Venetsanou Laboratory of Chemistry, Department of Food Science & Human Nutrition,School of Food, Biotechnology and Development, Agricultural University of Athens, 75 Iera Odos, 118 55 Athens, Greece
Eirini Anastasaki Laboratory of Chemistry, Department of Food Science & Human Nutrition,School of Food, Biotechnology and Development, Agricultural University of Athens, 75 Iera Odos, 118 55 Athens, Greece
Chrysavgi Gardeli Laboratory of Food Chemistry and Analysis, Department of Food Science & Human Nutrition,School of Food, Biotechnology and Development, Agricultural University of Athens, 75 Iera Odos, 118 55 Athens, Greece
Petros A. Tarantilis Department of Food Science & Human Nutrition, School of Food, Biotechnology and Development, Agricultural University of Athens, 75 Iera Odos, 118 55 Athens, Greece
Christos S Pappas Laboratory of Chemistry, Department of Food Science & Human Nutrition, School of Food, Biotechnology and Development, Agricultural University of Athens, 75 Iera Odos, 118 55 Athens, Greece

DOI:

https://doi.org/10.9755/ejfa.2016-11-1670

Keywords:

Antioxidant activity, ATR-FTIR, chemometrics, herbal infusions, PLS

Abstract

Antioxidant activity of 19 different mixed herbal infusion as it was calculated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays was correlated using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and chemometrics. The spectral region 1538- 843 cm-1 in 2nd derivative mode and the partial least squares (PLS) regression were used. The correlation coefficient, the root-mean-square error of calibration (RMSEC) and the root-mean-square error of prediction (RMSEP) for the DPPH assay were 0.97, 130 and 118 respectively. The corresponding values for the ABTS assay were 0.99, 148 and 121. The above results show that it is possible to estimate the antioxidant activity according to DPPH and ABTS assays of different mixed herbal infusions using ATR-FTIR spectroscopy. Furthermore the proposed methods are simple, rapid and economical.