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Correlations of flavonoids content and antioxidant activity in bee honey from Bosnia and Herzegovina

Authors

  • Ismet Tahirovića University of Sarajevo, Faculty of Science, Department of Chemistry, Zmaja od Bosne 33-35, 71000 Sarajevo, B&H
  • Harun Kurtagić b Federal Institute of Agriculture Sarajevo, Butmirska cesta 40, 71000 Sarajevo, B&H, c
  • Narcisa Smječanin University of Sarajevo, Faculty of Science, Department of Chemistry, Zmaja od Bosne 33-35, 71000 Sarajevo, B&H,

DOI:

https://doi.org/10.9755/ejfa.2023.v35.i4.3009

Abstract

In this study, it was performed identification and quantification of flavonoids (apigenin, chrysin, hesperetin, kaempferol, luteolin, naringenin, and quercetin) and flavonoid glycosides (rutin and vitexin) in total 49 samples of five different honey types from Bosnia and Herzegovina: meadow honey (MH, 22 samples), forest (FH, 10), acacia (AH, 7), chestnut (CH, 5), and heather honey (HH, 5). Additionally, evaluation of correlations between FC and total hydrophilic antioxidant score (antioxidant activity against both: ROO· + OH·) in supernatants (s) and in bulk (noncentrifuged) solution (b) of these honey types was performed. Moreover, correlations between flavonoids content (FC) and previously reported antioxidant activity against both peroxyl and hydroxyl free radicals (AC(ROO·) and AC(OH·)) for the same honey samples was examined. High performance liquid chromatography with photodiode array detector (HPLC-DAD) and isocratic elution mode was used as method of analysis. Flavonoids were extracted by solid phase extraction (SPE). The average contents of three flavonoids (chrysin, naringenin, and luteolin) in MH were statistically higher than in AH (p**<0.01). Also, the average content of naringenin in FH was statistically higher than in CH (p*<0.05). We observed a high (positive) linear correlation between FC and AC(ROO·) in s of four honey types (FH, AH, HH, CH) (R2=0.920). If we correlate FC and AC(ROO·)s of three honey types (FH, AH, HH), linearity is very high (R2=0.968), and for FH, AH, CH linearity is complete. The correlation between FC and AC(ROO·) in b of the same honey types is similar, but lower. The correlation does not exist between FC and AC(OH·) neither in s nor in b of five or four honey types, but for FC to both (AC(OH·)s and AC(OH·)b) of three honey types (FH, AH, CH), linearity is moderate (R2=0.732 and R2=0.696, respectively).

Keywords: antioxidant activity, correlations, flavonoids, honey, HPLC-DAD.

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Published

2023-03-01 — Updated on 2023-05-11

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How to Cite

Tahirovića, I., H. Kurtagić, and N. Smječanin. “Correlations of Flavonoids Content and Antioxidant Activity in Bee Honey from Bosnia and Herzegovina”. 2023. Emirates Journal of Food and Agriculture, vol. 35, no. 4, May 2023, doi:10.9755/ejfa.2023.v35.i4.3009.

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Research Article