Assessment of the effects of Annona muricata leaf aqueous extract in vitro

  • Buzogáňová Liliána 1Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 040 11 Košice, Slovak Republic
  • Kello Martin Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 040 11 Košice, Slovak Republic
  • Bago Pilátová Martina Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 040 11 Košice, Slovak Republic
  • Daniela Ogurčáková Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 040 11 Košice, Slovak Republic
  • Janka Vašková Faculty of Medicine, Pavol Jozef Šafárik University in Košice

Abstract

Background:  The extracts of Annona muricata, also known as graviola, form part of traditional medicine.


Objectives: Verification of their effects and elucidation of their mechanisms is beneficial in terms of the utility of these extracts in treatment or prevention.


Methods: We monitored the effectiveness of an extract of dried graviola leaves available for direct consumption on cancer cell lines and isolated rat liver mitochondria.


Results: The Jurkat cell line exhibited the highest, sensitivity, with the metabolic activity of cells reaching less than 10% at the highest tested concentration. The effect was dose-dependent. The viability of Jurkat cells was affected after more than the 72 hours. At the same time, there was also a reduction of mitochondrial membrane potential. At 24 to 48 hours incubation, no increase was found in the activity of caspase-3 but increased levels were found for the non-phosphorylated form of Bcl-2. Higher concentrations of graviola affected the mitochondrial redox state by dramatically reducing the levels of reduced glutathione. This was achieved through reduction in the activity of glutathione-S-transferase.


Conclusion: Our findings indicate that the studied preparation of graviola displayed cytotoxic effects on some cells, depending on the dose and length of action.


 

Author Biographies

Buzogáňová Liliána, 1Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 040 11 Košice, Slovak Republic

Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 040 11 Košice, Slovak Republic

Kello Martin, Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 040 11 Košice, Slovak Republic

Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 040 11 Košice, Slovak Republic

Bago Pilátová Martina, Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 040 11 Košice, Slovak Republic

Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 040 11 Košice, Slovak Republic

Daniela Ogurčáková, Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 040 11 Košice, Slovak Republic

Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 040 11 Košice, Slovak Republic

Janka Vašková, Faculty of Medicine, Pavol Jozef Šafárik University in Košice

Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Tr. SNP 1

 

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How to Cite
Liliána, B., K. Martin, B. P. Martina, D. Ogurčáková, and J. Vašková. “Assessment of the Effects of Annona Muricata Leaf Aqueous Extract in Vitro”. Emirates Journal of Food and Agriculture, Vol. 33, no. 11, Jan. 2022, doi:https://doi.org/10.9755/ejfa.2021.v33.i11.2785. Accessed 25 May 2022.
Section
Research Article