A FAST AND EFFICIENT METHOD TO DETERMINE THE POSITION OF ALIEN GENES IN TRANSGENIC PLANTS

Authors

  • Mahmood ur-Rahman National Centre of Excellence in Molecular Biology, Pakistan
  • S. Noreen
  • T. Husnain
  • S. Riazuddin

DOI:

https://doi.org/10.9755/ejfa.v22i3.4892

Keywords:

Bt genes, Fluorescence in situ hybridization, transgene, DNA probe

Abstract

Phenotypic variations are commonly observed in transgenic plants. Basmati rice (Oryza sativa L.) and cotton (Gossypium hirsutum), locally transformed with Bt genes, also exhibited such variations in their morphology. One possible cause of these variations might be the position of transgene on chromosome (s). An experiment was conducted to determine the position of the alien genes. It was determined by Fluorescence in situ hybridization (FISH) by hybridizing fluorescien labeled DNA probe on mitotic chromosomes of transgenic rice and cotton. An easy and simple protocol was developed as compared to available lengthy and difficult methods. The method is quick and efficient.

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Published

2017-10-25

How to Cite

ur-Rahman, M., S. Noreen, T. Husnain, and S. Riazuddin. “A FAST AND EFFICIENT METHOD TO DETERMINE THE POSITION OF ALIEN GENES IN TRANSGENIC PLANTS”. Emirates Journal of Food and Agriculture, vol. 22, no. 3, Oct. 2017, pp. 223-31, doi:10.9755/ejfa.v22i3.4892.

Issue

Section

Short Communication