Cytotoxic effect of multifruit polyphenol preparation on human breast cancer cell lines

  • Marta Ziaja Sołtys Department of Biology with Genetics, Medical University of Lublin, Chodźki 4A Street, 20-093 Lublin, Poland
  • Dominik Szwajgier Department of Biotechnology, Human Nutrition and Food Commodity Science, University of Natural Sciences, Skromna Street 8, 20-704 Lublin, Poland
  • Wirginia Kukuła- Koch Department of Pharmacognosy, Medical University of Lublin, Chodźki 1 Street, 20-093 Lublin, Poland

Abstract

Natural compounds are extensively used in the treatment of various diseases. Regular consumption of polyphenols plays an important role in the protection of health by reducing the risk of degenerative diseases, including cancer. The evaluation of the cytotoxic effect of the newly obtained multifruit polyphenolic preparation (composed of seven fruit) on T47D and MCF-7 breast cancer cells and MCF-12A normal cells. The PP was produced on the basis of combined ultrafiltrates obtained from chokeberry, raspberry, wild strawberry, apricot, peach, bilberry, and cranberry. The experiments were performed using human mammary gland cancer cell lines T47D (ductal cancer) and MCF-7 (adenocarcinoma) and normal breast cell line MCF-12A. Chromatographic techniques confirmed the highest contribution of cyanidin 3-O-glucoside, p-coumaroyl glucoside and chlorogenic acid in the PP. The PP exhibited dose-dependent cytotoxic effects towards MCF-7 and T47D cancer cell lines (IC50 = 1.2 μg . cm-3) and MCF-12A cells (IC50 = 0.6 μg . cm-3). The MTT cytotoxicity assay and microscopic observations confirmed the cytopathic effect of the PP on cell lines. It is supposed that berry polyphenols interfered with estrogen receptors leading to changes in the production of paracrine growth factors and therefore, PP was less cytotoxic towards the MCF-7 and T47D cell lines than against the MCF-12A cell line.

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How to Cite
Sołtys, M. Z., D. Szwajgier, and W. K.- Koch. “Cytotoxic Effect of Multifruit Polyphenol Preparation on Human Breast Cancer Cell Lines”. Emirates Journal of Food and Agriculture, Vol. 33, no. 4, May 2021, pp. 320-7, doi:https://doi.org/10.9755/ejfa.2021.v33.i4.2683. Accessed 28 Sept. 2021.
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Research Article