EVALUATION OF GENETIC VARIATION IN MUTANTS OF BLACK GRAM (VIGNA MUNGO L.) HEPPER) AS REVEALED BY RAPD MARKERS
Keywords:Gamma rays, Ethyl methane sulphonate, DNA polymorphism
One of the most possibilities is the genetic marker system to find out the genetic variation. The advent of large-scale DNA sequencing technology has generated a tremendous amount of sequence information for many important organisms. The genetic variation was evaluated in four mutants (high seed protein, tall, bushy and dwarf mutants) along with parent cultivar (control) by 20 random primers which generated 202 fragments scored with 58 polymorphic DNA bands. The average DNA bands were 10.1 per locus ranged from 1 to 9. The average polymorphic rates were 38.37 % among mutants and parent cultivar (control) through the 20 primers. Primer OPK–06 and OPK-11 revealed 62.5 % DNA polymorphism. Five genotypes were used to constructed dendrogram based on the similarity matrix, suggested that genetic distance from 0.621 to 0.785. The DNA variation might have been caused by mutation due to gamma rays and ethyl methane sulphonate. Hence, research is needed to analyse function of mutated genes for their mutants characters for future prospects because, such mutants and their genes when using in cross breeding/transgenic technologies will found more production in the development of improved crop varieties like high seed protein, lodging resistances, semi dwarf with high yield.