Effect of Rhodopseudomonas palustris G5 on seedling growth and some physiological and biochemical characteristics of cucumber under cadmium stress

Honglian Ge; Zhonghua Liu; Fuli Zhang

doi:10.9755/ejfa.2017.v29.i11.1327

Authors

Honglian Ge College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, China
Zhonghua Liu College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, China
Fuli Zhang College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, China

DOI:

https://doi.org/10.9755/ejfa.2017.v29.i11.1327

Keywords:

Rhodopseudomonas palustris; Cadmium; Cucumber seedlings; Physiological parameters; Antioxidant enzymes

Abstract

At present, we investigated whether Rhodopseudomonas palustris G5 strain treatments diminished the damage on cucumber seedlings caused by cadmium (Cd) stress. Growth and physiological parameters of cucumber seedlings were determinated at laboratory after co-treating seedlings with Rhodopseudomonas palustris G5 strain and different concentrations of Cd (50, 100, 150, 200, 300 mg L^-1). The results indicated that Cd stress significantly reduced shoot height, root length, total chlorophyll content, root activity, but increased malondialdehyde (MDA) content. In addition, lower concentrations of Cd stress increased soluble sugar and soluble protein contents, and improved the activities of superoxide dismutase (SOD; EC: 1.15.1.1) and peroxidase (POD; EC: 1.11.1.7), but higher concentrations of Cd decreased the soluble sugar and soluble protein contents, and inhibited SOD and POD activities. G5 strain application markedly reduced the toxic effects of Cd on cucumber seedlings. In sum, G5 strain treatment significantly alleviated damage on cucumber seedlings caused by Cd, and increased shoot height, root length, total chlorophyll content, root activity, the contents of soluble sugar and soluble protein, as well as the activities of SOD and POD, but decreased MDA content compared with non- treating plants.

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References

Akram, N. A. and M. Ashraf. 2013. Regulation in plant stress tolerance by a potential plant growth regulator, 5-Aminolevulinic acid. J. Plant Growth Regul. 32: 663-679.
Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
Cui, M. G., Y. C. Lin, Y. G. Zu, T. Efferth, D. W. Li and Z. H. Tang. 2015. Ethylene increases accumulation of compatible solutes and decreases oxidative stress to improve plant tolerance to water stress in Arabidopsis. J. Plant Biol. 58: 193-201.
El-Beltagi, H. S., A. A Mohamed and M. M. Rashed. 2010. Response of antioxidant enzymes to cadmium stress in leaves and roots of radish (Raphanus sativus L.). Not. Sci. Biol. 2: 76-82.
Fargašová, A. 2001. Phytotoxic effects of Cd, Zn, Pb, Cu and Fe on Sinapis alba L. seedlings and their accumulation in roots and shoots. Biol. Plant. 44: 471-473.
Gill, P. K., A. D. Sharma, P. Singh and S. S. Bhullar. 2003. Changes in germination, growth and soluble sugar contents of Sorghum bicolor (L.) Moench seeds under various abiotic stresses. Plant Growth Regul. 40: 157-162.
Gill, S. S and N. Tuteja. 2011. Cadmium stress tolerance in crop plants: probing the role of sulfur. Plant Signal. Behav. 6: 215-222.
Hegedüs, A., S. Erdei and G. Horváth. 2001. Comparative studies of H2O2 detoxifying enzymes in green and greening barley seedlings under cadmium stress. Plant Sci. 160: 1085-1093.
Horváth, G., M. Droppa, Á. Oravecz, V. L. Raskin and J.B. Marder. 1996. Formation of the photosynthetic apparatus during greening of cadmium-poisoned barley leaves. Planta. 199: 238-243.
Hotta, Y., T. Tanaka, H. Takaoka, Y. Takeuchi and M. Konnai. 1997. Promotive effects of 5-aminolevulinic acid on the yield of several crops. Plant Growth Regul. 22: 109-114.
Kerley, S. J. 2000. The effect of soil liming on shoot development, root growth, and cluster root activity of white lupin. Biol. Fertil. Soils. 32: 94-101.
Li, S., S. Zhang, X. Ding, X. Liao and R. Wang. 2013. Spraying silicon and/or cerium sols favorably mediated enhancement of Cd/Pb tolerance in lettuce grown in combined Cd/Pb contaminated soil. Procedia Environmental Sciences. 18: 68-77.
Li, Y., Q. Zhou, F. Li, X. Liu and Y. Luo. 2008. Effects of tetrabromobisphenol A as an emerging pollutant on wheat (Triticum aestivum) at biochemical levels. Chemosphere. 74: 119-124.
Lutts, S., J. M. Kinet and J. Bouharmont. 1996. NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Ann. Bot. 78: 389-398.
Mishra, B., R. S. Sangwan, S. Mishra, J. S. Jadaun, F. Sabir and N. S. Sangwan. 2014. Effect of cadmium stress on inductive enzymatic and nonenzymatic responses of ROS and sugar metabolism in multiple shoot cultures of ashwagandha (Withania somnifera Dunal), Protoplasma. 251: 1031-1045.
Naeem, M. S., M. Rasheed, D. Liu, Z. L. Jin, D. F. Ming, K. Yoneyama, Y. Takeuchi and W. J. Zhou. 2011. 5-aminolevulinic acid ameliorates salinity-induced metabolic, water-related and biochemical changes in Brassica napus L. Acta. Physiol. Plant. 33: 517-528.
Nunkaew, T., D. Kantachote, H. Kanzaki, T. Nitoda and R. J. Ritchie. 2014. Effects of 5-aminolevulinic acid (ALA)-containing supernatants from selected Rhodopseudomonas palustris strains on rice growth under NaCl stress, with mediating effects on chlorophyll, photosynthetic electron transport and antioxidative enzymes. Electron. J. Biotechnol. 17: 19-26.
Parys, E., E. Romanowska, M. Siedlecka and J. W. Poskuta. 1998. The effect of lead on photosynthesis and respiration in detached leaves and in mesophyll protoplasts of Pisum sativum. Acta. Physiol. Plant. 20: 313-322.
Popova, L. P., L. T. Maslenkova, R. Y. Yordanova, A. P. Lvanova, A. P. Krantev, G. Szalai and T. Janda. 2009. Exogenous treatment with salicylic acid attenuates cadmium toxicity in pea seedling. Plant Physiol. Bioch. 47: 224-231.
Pružinská, A., G. Tanner, I. Anders, M. Roca and S. Hörtensteiner. 2003. Chlorophyll breakdown: pheophorbide a oxygenase is a Rieske-type iron–sulfur protein, encoded by the accelerated cell death 1 gene. PNAS. 100: 15259–15264.
Sasaki, K., M. Watanabe, Y. Suda, A. Ishizuka and N. Noparatnaraporn. 2005. Applications of photosynthetic bacteria for medical fields. J. Biosci Bioeng. 100: 481488.
Xu, J., Y. Feng, Y. Wang, X. Luo, J. Tang and X. Lin. 2016. The foliar spray of Rhodopseudomonas palustris grown under Stevia residue extract promotes plant growth via changing soil microbial community. J. Soils. Sediments. 16: 916-923.
Xu, P., J. Zou, Q. Meng, J. Zou, W. Jiang and D. Liu. 2008. Effect of Cd2+ on seedling growth of garlic (Allium sativum L.) and selected physiological and biochemical characters. Bioresource Technol. 99: 6372-6378.
Wang, M. E. and Q. X. Zhou. 2006. Joint stress of chlorimuron-ethyl and cadmium on wheat Triticum aestivum at biochemical levels. Environ. Pollut. 144: 572-580.
Weckx, J. E. J. and H. M. M. Clijsters. 1996. Oxidative damage and defense mechanisms in primary leaves of Phaseolus vulgaris as a result of root assimilation of toxic amounts of copper. Physiol. Plant. 96: 506-512.
Wu, Y. X. and A. von Tiedemann. 2002. Impact of fungicides on active oxygen species and antioxidant enzymes in spring barley (Hordeum vulgare L.) exposed to ozone. Environ. Pollut. 116: 37-47.
Zarco-Tejada, P. J., A. Berjón, R. López-Lozano, J. R. Miller, P. Martín, V. Cachorro, M. R. González and A. de Frutos. 2005. Assessing vineyard condition with hyperspectral indices: leaf and canopy reflectance simulation in a row-structured discontinuous canopy. Remote Sens. Environ. 99: 271-287.