SEQUENCE AND POLYMORPHISM ANALYSIS OF THE CAMEL (CAMELUS DROMEDARIUS) MYOSTATIN GENE

  • Stefania Muzzachi Department of Biosciences, Biotechnologies, Biopharmaceutics, Viale Amendola 165/ 70126 Bari, Italy
  • Ahmad Oulmouden Unité de Génétique Moléculaire Animale, Université de Limoges Faculté des Sciences et Techniques, INRA UMR 1061, 123 Avenue Albert Thomas, 87060 Limoges, France
  • Youcef Cherifi Laboratoire de Génétique Moléculaire et Cellulaire, Faculté des Sciences de la Nature et de la Vie, Département de Génétique Moléculaire Appliquée. Université des Sciences et de la Technologie d'Oran Mohamed Boudiaf BP 1505 El Mnaouer, Oran, Algerie
  • Habib Yahyaoui Laboratoire Elevage et Faune Sauvage, Institut des Régions Arides, km 22.5 Route Djorf 4119 Medenine, Tunisie
  • Mohamed Ali Zayed Meat Technology Unit, Animal Breeding Department, Desert Research Center, 1 Mathaf El-Matarya St., El-Matarya, Cairo, Egypt
  • Pamela Burger Institut für Populationsgenetik, Vetmeduni Vienna, Veterinärplatz 1, 1210 Wien, Austria
  • Giovanni Michele Lacalandra Department of Emergency and Organ Transplantation (D.E.T.O.), Veterinary Clinics and Animal Production Section, University of Bari, Valenzano, Bari, Italy
  • Bernard Faye CIRAD-ES, Campus International de Baillarguet, 34398 Montpellier Cedex 1, France
  • Elena Ciani FAO/MOA Camel project, P.O.Box 761Al-Kharj, 11942 Saudi Arabia

Abstract

Myostatin (MSTN), a negative regulator of skeletal muscle development in mammals, represents a key target for genetic investigations in meat-producing animals, with mutations responsible for increased skeletal-muscle mass currently described in several livestock species. Dromedary camels play a major economic role as suppliers of meat for human consumption across several countries. Notwithstanding, a comprehensive characterization of the sequence variability at the Camelus dromedarius MSTN locus was still lacking. Here we present the first extensive sequence and polymorphism analysis of the MSTN gene in the C. dromedarius species. Out of more than 3.6 kb of nucleotide sequence screened on 22 animals from 3 different Northern African regions, only 3 variant sites in the first intron were detected. The low observed diversity may reflect the evolutionary history of the species, likely developed as domesticates from a low variable wild ancestor population. Sequence identity among C. dromedarius and other Cetartiodactyla highlighted a tree topology consistent with previous reports of a closer relationship between Tylopoda and Suiformes. A close similarity between C. ferus and C. dromedarius was observed within Tylopoda. A markedly higher sequence identity between C. dromedarius and the other vertebrate species was observed at the MSTN locus compared to other genes, thus confirming it as a highly conserved target across mammals.

Keywords: Camelus dromedarius, Myostatin, Sequence, Polymorphism
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How to Cite
Muzzachi, S., A. Oulmouden, Y. Cherifi, H. Yahyaoui, M. Zayed, P. Burger, G. Lacalandra, B. Faye, and E. Ciani. “SEQUENCE AND POLYMORPHISM ANALYSIS OF THE CAMEL (CAMELUS DROMEDARIUS) MYOSTATIN GENE”. Emirates Journal of Food and Agriculture, Vol. 27, no. 4, Apr. 2015, pp. 367-73, doi:https://doi.org/10.9755/ejfa.v27i4.19910. Accessed 8 Aug. 2020.
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Regular Articles