Assessment of the immunomodulatory effect of Aloe vera polysaccharides extracts on macrophages functions

  • Berenice Aranda-Cuevas
  • Jorge Tamayo- Cortez
  • Lourdes Vargas y Vargas Instituto Tecnológico de Mérida. Km 5 Carretera Mérida-Progreso S/N CP. 97118. Mérida, Yucatán, México
  • Ignacio Islas- Flores Centro de Investigación Científica de Yucatán, A.C. Calle 43 No. 130, Colonia Chuburná de Hidalgo CP 97200, Mérida, Yucatán, México
  • Víctor Arana- Argáez Laboratorio de Farmacología, Facultad de Química, Universidad Autónoma de Yucatán. Calle 43 613 x Calle 90, Colonia Inalámbrica, 97069 Mérida, Yucatán, México
  • Sara Solís-Pereira, Luis Cuevas-Glory Instituto Tecnológico de Mérida. Km 5 Carretera Mérida-Progreso S/N CP. 97118. Mérida, Yucatán, México
  • Carlos Hernán Herrera Méndez Agroindustrial Engineering Department, Campus Celaya-Salvatierra, Universidad de Guanajuato, Salvatierra, México

Abstract

The present study evaluates the immunomodulatory effect of high molecular weight fractions of Aloe vera polysaccharides harvested during the dry season (March-April) and the rainy season (August-September). Peritoneal macrophages (MΦs) secluded from Balb/c mice underwent treatment with A. vera leaves extract and acemannan standard (the major component found in A. vera) and stimulated with lipopolysaccharides (LPS). Macrophage cell viability was assessed by the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide method. Phagocytic activity was also evaluated in peritoneal macrophages, such as the production of nitric oxide and interleukin 6 (IL-6). In the results, found that the A. vera polysaccharides harvested during the rainy season stimulated the phagocytic activity with greater intensity than dry season and improvement NO and IL-6 production. No cytotoxic effect was found on cell viability and they cause a significant proliferative effect on macrophages in a concentration-dependent manner. It can be concluded that the A. vera polysaccharides harvested during the rainy season possessed a stronger immunostimulatory effect compared to the extracts from leaves obtained during dry seasons in a concentration-dependent manner without aff at the cell viability of macrophages.

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