Identification and antioxidant activity of carotenoids from superfine powder of Rhodobacter sphaeroides

  • Zuming Li Department of Food Science and Beijing Key Laboratory of Bioactive Substances and Functional Foods, College of Biochemical Engineering, Beijing Union University, Beijing, China
  • Lina Kong Department of Food Science and Beijing Key Laboratory of Bioactive Substances and Functional Foods, College of Biochemical Engineering, Beijing Union University, Beijing, China
  • Bodi Hui Department of Food Science and Beijing Key Laboratory of Bioactive Substances and Functional Foods, College of Biochemical Engineering, Beijing Union University, Beijing, China
  • Xiaoya Shang Department of Food Science and Beijing Key Laboratory of Bioactive Substances and Functional Foods, College of Biochemical Engineering, Beijing Union University, Beijing, China
  • Liping Gao Department of Food Science and Beijing Key Laboratory of Bioactive Substances and Functional Foods, College of Biochemical Engineering, Beijing Union University, Beijing, China
  • Na Luan Department of Food Science and Beijing Key Laboratory of Bioactive Substances and Functional Foods, College of Biochemical Engineering, Beijing Union University, Beijing, China
  • Xuliang Zhuang Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China
  • Dong Wang Wuxi Zhongke Huoli Biotechnology Co., LTD, Yixing City, Jiangsu Province, China
  • Zhihui Bai College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China

Abstract

The interest in carotenoids from the natural-antioxidant point of view has recently risen sharply because of their substantial health benefits. Here, we report the identification and antioxidant activity of carotenoids extracted by superfine grinding from Rhodobacter sphaeroides 3757 which is a species of photosynthetic bacteria. After separation and purification by silica gel column chromatography and reversed-phase high performance liquid chromatography (RP-HPLC), the four major carotenoids from the superfine powder of R. sphaeroide 3757 were identified as bixin, hydroxyspheroidenone, 3,3¢,4¢-tetrahydrospirilloxanthin-20-al, and spheroidenone by reversed phase - high performance liquid chromatography - atmospheric pressure chemical ionization - mass spectrometry (RP-HPLC-APCI-MS). The antioxidant activity of the carotenoids extracted after superfine grinding of dry biomass of R. sphaeroide 3757 was higher than that after ultrasonic treatment. When the ratio solvent-to-solid was 30, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity, reducing power, and lipid peroxidation inhibitory activity of the extracts after superfine grinding were 74.0% ± 3.1%, 0.497 ± 0.022, and 77.6% ± 3.2%, respectively. By contrast, the extracts after ultrasonic treatment, these numbers were 61.0% ± 2.5%, 0.328 ± 0.014, and 55.2% ± 2.3%, respectively. These results indicate that carotenoids from R. sphaeroide 3757 show a significant antioxidant activity in vitro in a concentration-dependent manner, and that superfine grinding is the optimal extraction method. We hope to provide scientific guidance for commercial production of natural- antioxidant and functional food from carotenoids of R. sphaeroide.

Keywords: Rhodobacter sphaeroides; Carotenoids; Superfine grinding; HPLC-DAD-APCI-MS; Antioxidant activity

References

Chi, S.C., D.J. Mothersole, P. Dilbeck, D.M. Niedzwiedzki, H. Zhang, P. Qian, C. Vasilev, K.J. Grayson, P.J. Jackson, E.C. Martin, Y. Li, D. Holten and C.N. Hunter. 2015. Assembly of functional photosystem complexes in Rhodobacter sphaeroides incorporating carotenoids from the spirilloxanthin pathway. BBA - Bioenergetics 1847: 189 - 201.
Chiu K.H. and W.S. Liu. 2014. Dietary administration of the extract of Rhodobacter sphaeroides WL-APD911 enhances the growth performance and innate immune responses of seawater red tilapia (Oreochromis mossambicus× Oreochromis niloticus). Aquaculture 418-419: 32-38.
Chiste, R.C., F. Yamashita, F.C. Gozzo and A.Z. Mercadante. 2011. Simultaneous extraction and analysis by high performance liquid chromatography coupled to diode array and mass spectrometric detectors of bixin and phenolic compounds from annatto seeds. J. Chromatogr. A 1218: 57 - 63.
Enzell, C.R., G.W. Francis and S. Liaaen-Jensen. 1969. Mass spectrometric studies of carotenoids 2. a survey of fragmentation reactions. Acta Chem. Scand. 23: 727 - 750.
Francis, G.W. and S. Liaaen-Jensen. 1970. Bacterial carotenoids: 33. carotenoids of thiorhodaceae: 9. the structures of the carotenoids of the rhodopinal series. Acta Chem. Scand. 24: 705 - 2712.
Fu, H.F., B.J. Xie, S.J. Ma, X.R. Zhu, G. Fan and S.Y. Pan. 2011. Evaluation of antioxidant activities of principal carotenoids available in water spinach (Ipomoea aquatica). J. Food Compos. Anal. 24: 288 - 297.
Giuffrida, D., P. Dugo, G. Torre, C. Bignardi, A. Cavazza, C. Corradini and G. Dugo. 2013. Characterization of 12 Capsicum varieties by evaluation of their carotenoid profile and pungency determination. Food Chem. 140: 794-802.
Kljak, K. and D. Grbeša. 2015. Carotenoid content and antioxidant activity of hexane extracts from selected Croatian corn hybrids. Food Chem. 167: 402 -408.
Lee, S.B., S.H. Yoo, P. Ganesan and H.S. Kwak. 2013. Physicochemical and antioxidative properties of Korean nanopowdered white ginseng. Int. J. Food Sci. Tech. 48: 2159-2165.
Li, X.M., W.H. Peng, Y.Y. Jia, L. Lu and W.H. Fan. 2016. Bioremediation of lead contaminated soil with Rhodobacter sphaeroides. Chemosphere 156: 228-235.
Liu, J.R., M.J. Chen and C.W. Lin. 2005. Antimutagenic and antioxidant properties of milk-Kefir and soymilk-Kefir. J Agr. Food Chem. 53: 2467 – 2474.
Liu, H.L., B.H. Chen, T.H. Kao and C.Y. Shiau. 2014. Carotenoids composition in Scutellaria barbata D. Don as detected by high performance liquid chromatography - diode array detection-mass spectrometry -atmospheric pressure chemical ionization. J. Funct. Foods 8C: 100 – 110.
Liu, S.L., G.M. Zhang, X.K. Li, P. Wu and J. Zhang. 2015. Enhancement of Rhodobacter sphaeroides growth and carotenoid production through biostimulation. J. Environ. Sci. 33: 21-28.
Ma, H.R., Y.D. Ma, Z.X. Zhang, Z.Y. Zhao, R. Lin, J.M. Zhu, Y. Guo and L. Xu. 2016. L-arginine enhances resistance against oxidative stress and heat stress in Caenorhabditis elegans. Int. J. Environ. Res. Public Health 13: 1-9.
Manwarning, J., E.H. Evans, G. Britton and D.R. Schneider. 1980. The identification of desmethyl – spheroidenone as a major carotenoid in aerobic cultures of Rhodopseudomonas Capsulata. FEBS Lett. 110: 47-49.
Manzano, P., J. Hernández, M. Quijano-Avilés, A. Barragán, I. Chóez-Guaranda, R. Viteri and O. Valle. 2017. Polyphenols extracted from Theobroma cacao waste and its utility as antioxidant. Emir. J. Food Agric. 29(1): 45-50.
Ramachandraiah, K. and K.B. Chin. 2016. Evaluation of ball-milling time on the physicochemical and antioxidant properties of persimmon by-products powder. Inno Food Sci Emerg Technol. 37: 115-124.
Rivera, S.M. and R. Canela-Garayoa. 2012. Analytical tools for the analysis of carotenoids in diverse materials. J. Chromatogr. A 1224: 1-10.
Sánchez, C.V., C. Serrano, M.C. Oliveira, P. Vasilenko, M. Santos and R.M. Sousa. 2016. Differential susceptibility of Morettini pears to blue mold caused by Penicillium expansum. Emir. J. Food Agric. 28(6): 374-380.
Sequeda-Castañeda, L.G., A.R. Barrera-Bugallo, C. Celis, J. Iglesias and L. Morales. 2016. Evaluation of antioxidant and cytotoxic activity of extracts from fruits in fibroblastoma HT1080 cell lines: four fruits with commercial potential in Colombia. Emir. J. Food Agric. 28(2): 143-151.
Silva, M.M. and F.C. Lidon. 2016. An overview on applications and side effects of antioxidant food additives. Emir. J. Food Agric. 28(12): 823-832
Sivathanu, B. and S. Palaniswamy. 2012. Purification and characterization of carotenoids from green algae Chlorococcum humicola by HPLC-NMR and LC-MS-APCI. Biomed. Prev. Nutr. 2: 276 - 282.
Stajcˇic´, S., G. C´etkovic´, J. Cˇanadanovic´-Brunet, S. Djilas, A. Mandic´ and D. Cˇetojevic´-Simin. 2015. Tomato waste: Carotenoids content, antioxidant and cell growth activities. Food Chem. 172: 225 - 232.
Takaichi, S., K. Furihata, K. Harashima. 1991. Light-induced changes of carotenoid pigments in anaerobic cells of the aerobic photosynthetic bacterium, Roseobacter denitrificans (Erythrobacter species OCh 114): reduction of spheroidenone to 3,4-dihydrospheroidenone. Microbiology 155: 473 - 476.
Takaichi, S. 1993. Usefulness of field desorption mass spectrometry in determining molecular masses of carotenoids, natural carotenoid derivatives and their chemical derivatives. Org. Mass Spectrom. 28: 785 - 788.
Venetsanou, A., E. Anastasaki, C. Gardeli, P.A. Tarantilis and C.S. Pappas. 2017. Estimation of antioxidant activity of different mixed herbal infusions using attenuated total reflectance Fourier transform infrared spectroscopy and chemometrics. Emir. J. Food Agric. 29(2): 149-155.
Wang, H.D., M. Zhang, G.H. Zhai and H. Jiang. 2012. Comparison of physicochemical and sensory quality of Lentinus edodes granular condiment prepared by different prilling and drying methods. Int. J. Food Sci. Tech. 47: 1265-1271.
Xia, Y.C., F. Bamdad, M. Gänzle and L.Y. Chen. 2012. Fractionation and characterization of antioxidant peptides derived from barley glutelin by enzymatic hydrolysis. Food Chem. 134: 1509 - 1518.
Yeliseev, A.A. and S. Kaplan. 1997. Anaerobic carotenoid biosynthesis in Rhodobacter sphaeroides 2.4.1:H20 is a source of oxygen for the 1-methoxy group of spheroidene but not for the 2-oxo group of spheroidenone. FEBS Lett. 403: 10 - 14.
Yoo, A.Y., M. Alnaeeli and J.K. Park. 2016. Production control and characterization of antibacterial carotenoids from the yeast Rhodotorula mucilaginosa AY-01. Process Biochem. 51: 463 - 473.
Zarza-García, A.L., E. Sauri-Duch, D. Raddatz-Mota, L.F. Cuevas-Glory, L.L. Pinzón- López, F. Rivera-Cabrera and J.A. Mendoza-Espinoza. 2017. Pharmacological, phytochemical and morphological study of three Mayan accessions of Bixa orellana L. leaves. Emir. J. Food Agric. 29(3): 163-169.
Zhao, X.Y., H.T. Zhu, G.X. Zhang and W.D. Tang. 2015. Effect of superfine grinding on the physicochemical properties and antioxidant activity of red grape pomace powders. Powder Technol. 286: 838-844.
Zhong, L., K.E. Gustavsson, S. Oredsson, B. Głab, J.L. Yilmaz and M.E. Olsson. 2016. Determination of free and esterified carotenoid composition in rose hip fruit by HPLC-DAD-APCI+-MS. Food Chem. 210: 541 - 550.
Zhu, K.X., S. Huang, W. Peng, H.F. Qian and H.M. Zhou. 2010. Effect of ultrafine grinding on hydration and antioxidant properties of wheat bran dietary fiber. Food Res. Int. 43: 943 - 948.
Zhuang, H., N. Tang and Y. Yuan. 2013. Purification and identification of antioxidant peptides from corn gluten meal. J. Funct. Foods 5: 1810 - 1821.
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How to Cite
Li, Z., L. Kong, B. Hui, X. Shang, L. Gao, N. Luan, X. Zhuang, D. Wang, and Z. Bai. “Identification and Antioxidant Activity of Carotenoids from Superfine Powder of Rhodobacter Sphaeroides”. Emirates Journal of Food and Agriculture, Vol. 29, no. 11, Oct. 2017, pp. 833-45, doi:https://doi.org/10.9755/ejfa.2017.v29.i11.1479. Accessed 22 Aug. 2019.
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