EPIDEMIOLOGICAL SURVEYS OF CAMEL TRYPANOSOMOSIS IN AL-JOUF, SAUDI ARABIA BASED ON PCR AND ELISA

  • Mohamed Elwathig FAO Camel project UTF/SAU/044/SAU, Al-Kharj, Saudi Arabia
  • Bernard Faye CIRAD, UMR SELMET, Montpellier F34398, France
  • Sophie Thevenon CIRAD, UMR INTERTRYP, Montpellier F34398, France
  • Sop Ravel CIRAD, UMR INTERTRYP, Montpellier F34398, France
  • Geraldine Bosssard CIRAD, UMR INTERTRYP, Montpellier F34398, France

Abstract

Trypanosomosis due to Trypanosoma evansi (surra) is a major enzootic disease of the dromedary camel. The present study was conducted to determine the prevalence of camel trypanosomosis in the northern part of Saudi Arabia with different methods of diagnosis (ELISA, PCR) and to compare the results to whose obtained previously with card Agglutination test (CATT/T.evansi). A total of 195 blood samples and 118 serum samples were used for molecular and serological investigation respectively. After analyses, 25% (49/195) and 3% (4/118) samples were positive using PCR and ELISA respectively. The variability of trypanosomosis was highly significant to the factor moving, location, breed and clinical signs with PCR. The discrepancy between PCR, CATT test and ELISA is likely due to antibodies degradation on spotted papers maintained several at ambient temperature. This is the first molecular diagnosis report, which gives a picture of camel trypanosomiasis in Al-jouf, Saudi Arabia.

Keywords: Trypanosoma evansi, camels, PCR, ELISA, Risk Factors, Al-jouf
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How to Cite
Elwathig, M., B. Faye, S. Thevenon, S. Ravel, and G. Bosssard. “EPIDEMIOLOGICAL SURVEYS OF CAMEL TRYPANOSOMOSIS IN AL-JOUF, SAUDI ARABIA BASED ON PCR AND ELISA”. Emirates Journal of Food and Agriculture, Vol. 28, no. 3, Nov. 2017, pp. 212-6, doi:https://doi.org/10.9755/ejfa.2015-09-759. Accessed 22 Feb. 2018.
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Regular Article